Enzyme Lab: Virtual Enzyme Kinetics
Rapport de stage : Enzyme Lab: Virtual Enzyme Kinetics. Recherche parmi 300 000+ dissertationsPar Green Silver • 20 Janvier 2021 • Rapport de stage • 396 Mots (2 Pages) • 436 Vues
Enzyme Lab: Virtual Enzyme Kinetics
Khoa Nguyen
40078865
Summary (conclusions):
An enzyme (ref. PR/05-210-101) has been assigned.
The optimum pH for the enzyme to function is 7
[pic 1]
The azide inhibits the catalytic process of the enzyme because it acts as a noncompetitive inhibition. To explain, azide binds to an enzyme somewhere other than the active site. Then, enzyme's three-dimensional structure is changed; its active site can still bind substrate with the usual affinity, but is no longer in the optimal arrangement to stabilize the transition state and catalyze the reation. The dissociation constant for an enzyme -inhibitor complex:
[pic 2]
From: Isbu.ac.uk, Enzyme Inhibition, 2014
The precision of the measurements for the rates was seen by a standard deviation of 0.082 for the enzyme without the inhibitor. For the measurements taken for the enzyme and inhibitor the precision of the tests were seen by a 0.032 standard deviation. The precision did vary with the different rates seen because of the presence or lack of presence of the inhibitor.
Results
Without Inhibitors
Solute Effect | pH Effects | Precision | |||||
[S] (nM) | Vo (umol/min) | pH | Vo (umol/min) | Trail | Vo (umol/min) | ||
0.3 | 0.3863 | 4 | Too slow | 1 | 3.34 | ||
50 | 4.5464 | 5 | 0.4248 | 2 | 3.30 | ||
0.43 | 0.7837 | 6 | 2.425 | 3 | 3.16 | ||
1.28 | 1.6646 | 7 | 4.2934 | 4 | 3.24 | ||
4.27 | 3.1459 | 8 | 4.266 | 5 | 3.16 | ||
12.8 | 4.234 | 9 | 2.1431 | ||||
Variable | Constant | Variable | Constant | Variable | Constant | ||
[S] | pH, [I], Volume | pH | [S], [I], Volume | [S], [I], pH, Volume |
Estimated Km: 2.13 🡪 Sample calculation: S ((V50/V0.1) – 1) = 0.2((4.55/0.39) -1) = 2.13
Actual Km: 2.26 +/- 0.178
Vmax: 4.77 +/- 0.194
[pic 3]
Precision: Mean of 3.240 μmol/min with a Standard Error of 0.082 μmol/min
[pic 4]
With Inhibitors
Solute Effect | pH Effects | ||||
[S] (nM) | Vo (umol/min) | pH | Vo (umol/min) | ||
0.43 | Too slow | 6 | 0.722 | ||
0.75 | 0.2723 | 6 | 0.7244 | ||
1.28 | 0.3852 | 6 | 0.6673 | ||
| 0.7214 | 6 | 0.7173 | ||
12.8 | 0.9428 | 7 | 1.2274 | ||
6.4 | 0.7617 | 7 | 1.2167 | ||
10 | 0.882 | 7 | 1.2021 | ||
7 | 1.2209 | ||||
7 | Too slow | ||||
7 | 1.2144 | ||||
7 | 1.1416 | ||||
Variable | Constant | Variable | Constant | ||
[S] | pH, [I], Volume | pH | [S], [I], Volume |
...